Sri is currently on leave at Octant, and we are not accepting new applicants/researchers.
Our laboratory develops methods to vastly increase our ability to empirically explore relationships between DNA sequence and biological function. We do this by developing and combining three related technologies: DNA synthesis, DNA sequencing, and genome engineering. First, we develop methods to build thousands to millions of designed DNA sequences using low-cost DNA microarrays. Second, we develop new ways to link the measurement of some biological function to an output that can be measured in multiplex using next-generation sequencing. Combining the two lets build large numbers of designs/hypothesis regarding a biological function, and testing them all at once in a multiplexed assay. Finally, using new genomic engineering technologies, we can do these large-scale synthesis/sequencing experiments in a wide-variety of cell types and organisms.
Besides this central theme, each of the projects is otherwise seemingly different. We work on organisms such as bacteria, yeast, and human cell lines, and on biological problems that span population genetics, evolution, gene regulation, protein structure-function, and neuroscience. Please see the members page to see what individuals are working on, or the publications page for our latest manuscripts.
Our laboratory develops methods to vastly increase our ability to empirically explore relationships between DNA sequence and biological function. We do this by developing and combining three related technologies: DNA synthesis, DNA sequencing, and genome engineering. First, we develop methods to build thousands to millions of designed DNA sequences using low-cost DNA microarrays. Second, we develop new ways to link the measurement of some biological function to an output that can be measured in multiplex using next-generation sequencing. Combining the two lets build large numbers of designs/hypothesis regarding a biological function, and testing them all at once in a multiplexed assay. Finally, using new genomic engineering technologies, we can do these large-scale synthesis/sequencing experiments in a wide-variety of cell types and organisms.
Besides this central theme, each of the projects is otherwise seemingly different. We work on organisms such as bacteria, yeast, and human cell lines, and on biological problems that span population genetics, evolution, gene regulation, protein structure-function, and neuroscience. Please see the members page to see what individuals are working on, or the publications page for our latest manuscripts.